| RADIATION STUDIES
VOL. 8"Radiation Studies" Volume VIII includes
the reports of VI Conference of Georgian radiobiologists, devoted to Centennial of
Radiobiology and the 150th anniversary of one of its founders - I.Tarkhan-Mouravi
(Tarchanoff)'s birth. Collection presents the periodic edition of the Scientific Research
Centre of Radiobiology and Radiation Ecology of The Georgian Academy of Sciences and
Georgian Academy of Ecological Sciences. Presented materials deal with the guidelines in
modern radiobiology, accomplished on different levels of biological system organizations
from molecular to population level. It includes also material on life, scientific and
public activity of I.Tarchan-Mouravi (1846-1905).
Contents
CENTENNIAL OF RADIOBIOLOGY ON THE 150TH ANNIVERSARY OF I.TARKHAN-MOURAVI
(TARCHANOFF)'S BIRTH ACTION OF SIGNAL X-IRRADIATION IN SUBLETHAL DOSES ON HEART RATE
VARIABILITY(HRV) AND ECG IN MALE CHINCHILLA RABBITS THE EFFECT OF IONIZING RADIATION ON AGGRESSIVE BEHAVIOR IN RATS,
PHISIOLOGICAL AND NEUROCHEMICAL CORRELATES MODIFICATION OF BEHAVIOR, BIOGENIC AMINES AND FREE AMINOACIDS CONTENT IN
THE BRAIN, AND RADIOSENSITIVITY INDUCED BY PARA-CLOROPHENYLALANINE (PCPA) IJECTION IN RATS EFFECT OF POWER FREQUENCY (50 HZ) ELECTROMAGNETIC FIELDS ON ELABORATION
STORAGE OF PASSIVE AVOIDANCE REACTION IN RATS STUDY OF COMBINED ACTION OF IONIZING RADIATION AND A STHESS FACTORS ON THE
RED BLOOD SYSTEM Communication I STUDI OF THE RED BLOOD REGULATION MECHANISMS UNDER
PHYSIOLOGICALLY NORMAL CONDITIONS AND SHORTLY AFTER IMMOBILIZATION STRESS ACTION OF THE WHOLE-BODY X-IRRADIATION IN SUBLETHAL AND SUPERLETHAL DOSES
ON SPONTANEUS AND INDUCED AGGRESSIVE BEHAVIOR IN RATS ABOUT RADIOPROTECTIVE PROPERTIES OF ALKANETHIOLS AND DIALKYLSULFIDES TRENDS OF DEVELOPMENT OF RADIOBIOLOGICAL STUDIES IN GEORGIA INVESTIGATION OF Mg, Cu AND Zn DISTRIBUTION IN CHILDREN'S HAIR BY
ATOM-ABSORPTION METHOD ON SOME PROBLEMS AT INSTRUMENTAL NEUTRON ACTIVATION ANALYSIS (INAA) OF
BIOLOGICAL MATERIALS PHYSICAL METHODS FOR DETERMINATION OF GOLD CONTENT IN ORES AND
CONCENTRATES THE INFLUENCE OF GAMMA-RADIATION ON PROTEINS COMPOSITION OF CALLUS
TISSUES ON INITIAL STAGE OF IT'S DIFFERENTIATIATION THE CREATION AND APPLICATION OF THE LABORATORY COMPLEXES OF
HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS ESTIMATIONS OF BACKGROUND RADIATION AND DOSE LOADINGS OF POPULATION IN
GEORGIA ESTABLISHMENT OF EXPOSURE DOSES OF GROUND RADIOACTIVITY IN THE TERRITORY
OF GEORGIA TECHNOGENICALLY MODIFIED RADIATION BACKGROUND - A YGIENIC ASPECT THE QESTION OF RADIATION BY RADON AMONG THE POPULATION OF GEORGIA POPYLLATION OF FOOD STUFF AND RATION WITH RADIONUCLIDE AS A RESULT OF
CHERNOBIL ACCIDENT. CREATION OF INFORMATION BASE ON RADIOHYDROGEOECOLOGICAL ANOMALIES OF
GEORGIA RADIATION-HYGIENIC ESTIMATION AND EPIDEMIOLOGIC ANALYSIS OF POPULATION
IRRADIATION LEVELS IN THE TRANSCAUCASUS CENTENNIAL OF RADIOBIOLOGY ON THE 150TH
ANNIVERSARY OF I.TARKHAN-MOURAVI (TARCHANOFF)'S BIRTH ABSTRACT. The article is devoted to the life, scientific and public activity of renowned Georgian scientist I.Tarchan-Mouravi (1846-1907), the author of a number of known discoveries in the field of biomedicine, including skin-galvanic reflex (Tarchanoff’s reflex). To him belong also pioneer studies in the field of biological action of X-rays, the results of which were published several months after the great discovery of William Konrad Roentgen, and were devoted to the problems of neuroradiobiology, radiation embryology, radioecology, etc. Being a century old, many problems explored by him are still of great topical interest. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Phisiology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 5-26, Article in English ACTION OF SIGNAL X-IRRADIATION IN SUBLETHAL DOSES ON HEART RATE
VARIABILITY(HRV) AND ECG IN MALE CHINCHILLA RABBITS ABSTRACT. Heart rate(HR) was one of the first criteria used to stady the effect of X- rays on biological processes. These studies were made in physiological laboratories of St.Peterburg University and Imperial Medico-Surgery Military Academy some mounts after the discovery of X-rays by William Konrad Roentgen. The autor of the investigation was a well- knowen Georgian physiologist Ivane Tarkhan-Mouravi(Tarñhanoff). These very investigatigations that laid the foundation to radiobiology as a field of science. The pioneer studies of Tarkhan-Mouravi deal with neuroradiobiology, radiation embryology, phytoradiobiology, radiation ecology and other trends. In order to study the effect of X-rays on on the metabolism and excitatory processes I.Tarkhan-Mouravi used, apert from many biological objects, in situ working frog heart preparation as well. No changes were observed in the heart contraction rate and intensity within the dose ranges used by him, while frogs spinale reflexes and skin melanofors activity, frogs and insects behaviour was sygnificantly modificated, and fish embrios development wholly depressed . In this opinion, chronotropic and bathmotropic shifts were not detected only bicause of the "X-ray source being not sufficiently potent". It should be emphasized that investigations by I.Tarkhan-Mouravi are the first experimental findings about the effect of ionizing radiation on cardiac function and generally about its biological action. Since then in the course of past certury a lot of studies dealt with these problems. Radiobiology has become on self-sufficient field of science, while study of the action of radiation on cardiac function is now one of its trends referred to as radiation cardiology. In spite of a century-old history the questions of the action of radiation on cardiac function heve not been ascertained so far. The paper is devoted to one of the important aspects of this problem, namely, the effect of sublethal doses of ionizing radiaton on the HR regulating mechanisms. Main parameters of time-domain(TD) and frequency-domain(FD) analysis of HRV, including power spectral density(PSD) within the range 2.5-0.015 Hz, beat-to-beat analysis, covariation and so on were studied in male chinchilla rabbits during passive wakefulness before and during 3 months after sublethal (0.5, 1.0 and 2.0 Gy) total X-irradiation. By comparison of the results of TD analysis and their derivative indices, as well as main parameters of spectral analysis two groups of rabbits with various initial level of regulation of cardiac activity and radiosensivity were distinguished: group I with the predominance of cholinergic system activity and group II with the predominance of adrenergic one, relatively more sensitive to X-rays. At the dose of 2.0 Gy changes of HRV are more pronounced and prolonged in the II group of animals. With relatively low probability (0.05 <P< 0.1) there was tendency to analogous changes after 0.5 and 1.0 Gy irradiation. After 24h of 2.0 Gy X-irradiation heart rate, amplitude of mode(Amo), tension index(TI), index of vegetative balance(VB), adequacy of regulation processes(ARP) and vegetative index of rhythm(VIR) were reliably increased (P<0.01-0.001), but the mode(Mo), standard deviation (STD) and range of variability(DX) were decreased(P<0.05). During first 3 days ratios of PSD in high frequencies sharply increased in both groups of animals while within the range of low frequencies(LF) - regressed. LF regression is especially distinct in II grouop of rabbits. Similar alterations prolonged 2 weeks, then the state was relatively stabilized, but in the second group of rabbits Amo, TI, VB, APR and VIR were decreased from 2 weaks to 2 months. At the end of 3 months the parameters of HRV were not statistically authentic difference from initial parameters in both groups of animals or differeces have relatively low probability (0.05 <P< 0.1). Analogous changes were observed in the PSD criteria. After 0.5, 1.0 and 2.0 Gy X- irradiation ECG-changes were not found during the first 3 mounths. Therefore, the early rhythmocardiological changes according to the phases of acute radiation injury; that is 1-3 days are the first reaction phase, 3-14 days are - latency phase, 2 weeks-1 month for I group of rabbits and 2 weeks-2 months for II group - the initial period of recovery. It is known that after irradiation long-lasting hypotensia and bradycardia develop in survived animals. This may be caused by alteration of cholinergic part of cardio-vascular regulatory system as a whole or by decrement of adrenergic one. Our rhythmocardiological studies suggest with great probability that the late effects of cardiac rhythm regulation abnormalities in sublethally irradiated rabbits result from insufficiency of adrenergic regulatory mechanisms. Details of this finding will be discussed in subsequent publications. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Phisiology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 27-66, Article in Georgian THE EFFECT OF IONIZING RADIATION ON AGGRESSIVE BEHAVIOR IN RATS,
PHISIOLOGICAL AND NEUROCHEMICAL CORRELATES ABSTRACT: Studies of conditioned passive avoidance reaction (CPAR) in aggressive and nonaggressive rats have demonstrated that fear reaction in the former persists longer than in the latter. However in aggressive rats conditioned active avoidance reaction (CAAR) is difficult to be elaborated and more number of combinations of conditioned and unconditioned signals to achieve the required criteria. After total irradiation in the dose 7Gy the number of aggressive animals maintaining fear reaction is reduced. Quantitative indices of CAAR elaboration in aggressive and nonaggressive animals exposed to 7Gy do not vary much from preirradation data, and in some cases aggressive animals show a weak tendency to the facilitation of conditioned reflex elaboration. A single X-iradiation in the range of 5,5 - 12,0 Gy results in a more considerable shortening of life-span in aggressive animals compared to nonagressive one. It was established that LD 50/30 for aggressive animals equals to 6,10±0,058 Gy, and for nonagressive to 6,50±0,001. This difference is statistically significant with high probability (P<0,001). In aggressive rats amount of serotonin (5-HT), as well as total and nonproteinous sulfhydryl groups in various brain regions and specific binding of 5-HT are substanctially lower than in nonagressive ones.Irradiation in superlethal doses (50, 100 and 200 Gy) elicits phasic changes in behavior: first occurs loss of aggressiveness, then its temporary recovery and finally, irreversible suppression of aggressiveness and perish of the animals. The higher the iradiation dose the shorter are these cycles. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Neuroblolgy "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 67-78, Article in English MODIFICATION OF BEHAVIOR, BIOGENIC AMINES AND FREE AMINOACIDS CONTENT
IN THE BRAIN, AND RADIOSENSITIVITY INDUCED BY PARA-CLOROPHENYLALANINE (PCPA) IJECTION IN
RATS ABSTRACT: Intraperitoneal injection of pCPA(400 mg/kg) causes changes in the qantitative distribution of biogenic amines (BA), and free amino acids (FA) in olfactory bulbes, hippocampus, and hypothalamus, of the rats. 36-54 hours after injection of pCPA content of serotonin (5-HT) in olfactory bulbes, hypothalamus and hippocampus, drops and noradrenalin (NA-5-HT) retio increases. The study of pCPA influence on distribution of FA in the cerebral structures showed significant changes 36-54 hours after the drug injection. In the olfactory bulbes, hippocapus, hypothalamus statistically reliable increase of the ratio of aspartate- glutamate to asparagine-glutamate in observed. Intraperitoneal injection of pCPA causes facilitation of conditioned active avoidance and aggravation of passive avoidance. Besides, injaction of pCPA and immediate irradiation rats after 4-36 hours manifest aggression that retains till the end of life. This can be accounted for by radioprotective effect of twin-80. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Neuroblolgy "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 79-92, Article in Georgian EFFECT OF POWER FREQUENCY (50 HZ) ELECTROMAGNETIC FIELDS ON ELABORATION
STORAGE OF PASSIVE AVOIDANCE REACTION IN RATS ABSTRACT: The effect of single whole-body exposure to power-frequency (50 Hz) electromagnetic field (EMF) at induction of 1.5 mT and 3.6 mT on the elaboration and storage of passive avoidance reaction in rats was studied. One hour exposure to EMF at induction of 1.5 mT was shown to cause a disturbance in the previously elaborated passive avoidance reaction and to hamper the elaboration and memorizing of this reaction after exposure. Two hour exposure at induction of 3.6 mT causes relatively small, but statistically reliable decrement of the previously elaborated passive avoidance reaction and significantly hampers its elaboration and storige. The results obtained indicate the substantial biological activity of power-frequency electromagnetic fields, which alters rats’ behaviour and their memory. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Neuroblolgy and Radiation Phisiology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 93-104, Article in Georgian STUDY OF COMBINED ACTION OF IONIZING RADIATION AND A STHESS FACTORS ON
THE RED BLOOD SYSTEM The dynamics of population content of the peripheral blood erythrocytes (PBE) under normal physiological conditions and shortly after immobilization stress was studied in rats by an original methodological approach. As population spectrum in the given approach is considered the distrbution of the number of circulatory erythrocytes by their volume (V) and the extent of their spherulation (Q) characterizing the deformed cells and considered as their biological age. A body of mathematics affording, on the basis of dynamics of population spectrum, to evaluate values of erythrocyte stream arriving in and eliminatig from the bed is offered. Immediately after the impact of stress factor a dramatic increase in the number of circulatory erythrocytes and hematocrit was observed as judged by arriving in circulation of deposited erythrocytes. It has been established that the blood depot contains only young (easily deformable) erythrocytes. This testifies that old cells and erythrocytes of intermediate age are not being deposited. During the first 48 hours after the impact of a stress factor a dramatic reduction of the number of circulating erythrocytes end hemaocrit is observed. If in the first 24hr this is due to a sharp increase in the intensity of elimination, then in the period between 24-48hr. the process of the cell redeposition appears to be involved in it. It has been revealed that mainly the old cells and partially the cells of intermediate age are being eliminated. In the period between 3 and 18 days after exposure to a stress factor number of PBE and hematocrit, although being stabilized, remains on a low level. In this period one can observe leveling of the cell stream arriving in and eliminating from the bed, their intensity, houwever, being actually two-fold lower the control level. It is supposed to be due to a decrease of decrement of the spleen compression rather than attenuation of erythropoiesis, since at this period one can observe arrival in bed of erythrocytes of enlarged sizes suggesting a considerable tension of erythropoiesis. At the same time on the 20th day after the effect of stress factor the number of cells arriving in bed sharply increases. It has been established that in the process of aging the cell volume does not alter or alters but insignificantly. This indicates that during the erythrocyte life cycle a change of cell deformability is due mainly to a decrease in their surface area – fragmentation. The process of the cellular membrane fragmentation was shown not to be a monotunous process. Probability of erythrocyte availability in circulation was shown to be dependent on their deformability andsizes, this dependence being of saccadic character. In particular, the existence of a certain limit value of Qmax at which cells are effectively eliminated was found. At the same time if for erythrocytes with volum V*60*3 * Qmax=0.41, then for erythrocytes with V>60*3 *Qmax=0.37. We have found a strict correlation between the cell stream of arriving in and eliminating from the circulating bed. Various plausible schemes of identification and elimination of old erythrocytes were considered. In right of the data obtained the most plausible model seems to be selection of cells filtering by the spleen capillaries in terms of their geometrical characteristics. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Oncological Research Centre Health Service Ministry of Georgia Departtament of Cell Radobiology and Radiation Neuroblolgy "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 105-136, Article in Russian ACTION OF THE WHOLE-BODY X-IRRADIATION IN SUBLETHAL AND SUPERLETHAL
DOSES ON SPONTANEUS AND INDUCED AGGRESSIVE BEHAVIOR IN RATS ABSTRACT: The action of single exposure of the whole-body X-irradiation in sublethal (1.75 and 3.5 Gy) and superlethal (100 and 200 Gy) doses on spontaneous and induced by para-Chlorphenylalanine (pClPA) aggressive behavior in rats and their ethological characteristics were studied in Open Field(OF). Exposure of mice-killer rats to sublethal doses resulted in the loss of predatory aggression 15-18 days after irradiation with 1.75 Gy and on 5 - 9th day with of 3.5 Gy. Elimination of aggressivness and enhancement of emotionality on the one hand and decrement of locomotor and orientation-exploratory activity, on the other hand, were also indicated. The pClPA induced aggressiveness, if the drug was administered 10 min prior to irradiation or during the first three days after exposure to 3.5 Gy or during the first two weeks after exposure to 1.75 Gy was virtually unaltered: muricidity was revealed and dissappeared in the same periods as in control animals. The induced aggressiveness was considerably modified if pClPA was injected on the 18th day after exposure to 1.75 Gy and on the 10 th day after 3.5 Gy dose when in control rats aggressiveness was eliminated. Exposure of superlethal doses led to the suppression of aggressiveness no later than 3h after 100 Gy dose and 1h at 200 Gy. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Phisiology and Radiation Neuroblolgy "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 137-147, Article in English DYNAMICS OF BEHAVIOR AND NEUROCHEMICAL CORRELATES AT CHRONIC
INFORMATION OVERLOADING OF RATS HAVING UNDERGONE EMBRYONAL AND POSTNATAL DEVELOPMENT IN
HYPOMAGNETIC ENVIRONMENT ABSTRACT. Under conditions of contemporary development of the level of civilization entailing essential changes in the environment of human habitation, the problem of interrelation of the organism and external world becomes especially topical. Attenuated or hypomagnetic field is one of the products of human activity and proceeding from the reported evidence, represents rather an unfavourable factor of the environment. Especially this concerns the developing organism that does not yet possess adaptational mechanisms which protect the organism from the unfavourable influences of exogenic factors. We have made an attempt to evaluate resistance of the CNS to informational overloadings in rats having undergone embryonal and early postnatal development in hypomagnetic field in model experiments of conditioned reflex activity. A hypomagnetic field was created by means of three-component coil of low magnetic induction on the principle of diversely directed magnetic field to the vectors of earth magnetism. It has been shown that task acquisition in experimental rats is more rapid, the level of correct responses is higher in them. Visual observation on the rats' behavior in the maze has shown that experimental rats as compared to the control are more exultant, they mostly work in an upright position, very frequently their reaction to a conditioned stimulus is instant, coefficient of variation is considerably stable. Experimental rats, however, appeared more resistant to chronic informational overloadings, evidenced by their far rapid fatigue. Neurochemical analysis with determination of catecholamine concentration has revealed that in experimental rats noradrenaline and dopamine increase only in the hippocampus that indicates sensitivity of catecholaminergic neurons to the hypomagnetic factor. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Phisiology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 156-164, Article in English ABOUT RADIOPROTECTIVE PROPERTIES OF ALKANETHIOLS AND DIALKYLSULFIDES ABSTRACT: The low-temperature radiolysis of alkanethiols and dialkylsulfides has been investigated in individual state and in binary mixtures with hydrocarbons and alcohols.It is established that sulphurcontaining functional group protects the hydrocarbon part of the molecule from radiation effect. In pure thiols and sulfides this effectis of intramolecular kharacter. In binary mixtures with alkanes and alcohols the effective transfer of ionization and excitation energy from solvent molecules to the molecules of sulphur acceptor takes place. The efficiency of the protective action of alkanethiols ddand dialkylsulfides has been estimated. Georgian Academy of Sciences Inorganic Chemical and Electrochemical Institut Departtament of Radiochemistry "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 165-172, Article in English TRENDS OF DEVELOPMENT OF RADIOBIOLOGICAL STUDIES IN GEORGIA In dissertation, particularly in their subject-matter, earlier than anywhere else find a reflection new ideas and directions of studies. A collective nature of scientific activity manifests itself best of all while defining dissertation themes. The theses themselves pass through a multi- stepped various expertise. Array of abstracts of thesis contains substantal volume of information. This concerns both the information about the value, structure and composition of that scientific discipline materials of which served for their preparation and the information about the existence of scientific schools, scientific-leaders, various communication relationsh amongst the researchers. Therefore it can serve as an information base for detecting the trends of development of science as a whole, its branches or separate disciplines. The paper and appendix give an idea of general tendencies of development of radiobiological science in Georgia. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiobiological Informatics and Radiation Phisiology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 173-188, Article in Russian INVESTIGATION OF Mg, Cu AND Zn DISTRIBUTION IN CHILDREN'S HAIR BY
ATOM-ABSORPTION METHOD ABSTRACTS: The investigation of the concentration of metals in human hair and their distribution according to its length is considered as one of the reliable method of environment monitoring. The paper presents the results of the investigation of Mg,Cu and Zn distribution in healthy children's hair on the basis of the analysis carried out by atom absorption method.These date have a repair value. Georgian Academy of Sciences Institut of Fisics "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 189-192, Article in English ON SOME PROBLEMS AT INSTRUMENTAL NEUTRON ACTIVATION ANALYSIS (INAA) OF
BIOLOGICAL MATERIALS ABSTRACT: To carry out instrumental neutron activation analysis(INAA) of biological materials, samples go through the stages of preparation for irradiation itself in a channel of the nuclear reactor (NR). Each of these processes has its special characteristics. During INAA of biological materials it is desirable to observe three aspects given below: the comparison of element content in the biological samples dried at various temperatures was carried out; the change of mass of biological samples and comparison standards in dependence on the power of nuclear reactor and the time of irradiation in ''common''channels and in the model experiments were determined,and the influence of conditions of the reactor irradiation of the materials under investigation on the value of trace elements concentration was studied. Georgian Academy of Sciences Institut of Fisics "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 193-206, Article in English PHYSICAL METHODS FOR DETERMINATION OF GOLD CONTENT IN ORES AND
CONCENTRATES ABSTRACT: The analytikal possibility of two physical methods X-ray fluorescence (XFA) and Neutron Activation Analysis (NAA) has been estimated for determination of gold in ores and concentrates. In the case of XFA method two isotopic sources of 109Cd were used, each having the activity of 2.108 Bq. For the NAA method subcritical assembly enrichment with 235U isotope was used, allowing to obtain stable fluxes of thermal neutrons 107 neutron/cm2s. It was shown that simultaneous usage of these two methods allows to get both high expressivity (XFA) and high selectivity, sensitivity and precision (NAA) of gold determination. In this case the sensitivity of gold determination is up 0,1 ppm. Georgian Academy of Sciences Institut of Fisics "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 207-213, Article in English THE INFLUENCE OF GAMMA-RADIATION ON PROTEINS COMPOSITION OF CALLUS TISSUES ON INITIAL
STAGE OF IT'S DIFFERENTIATIATION ABSTRACT: At present moment the use of radiobiological methods, that effects on various vital functions processes in plant organizm acquires an important scientific and practical meaning. First of all, it is caused by active use of the radiation factor as an opportunity, that allows to research various structure-functional levels of plant's organization under extremal conditions. The practice of these experiments showed, that by this method it is possible to obtain very interesting effects for further theoretical aspects improvement of radiation influences on plant and for concrete applied tasks solution. Exactly to it one can applie the studies of gamma- radiation's effect on cytodifferentiation and constituted processes. Georgian Academy of Agricultural Siences Scientific-research Institute of agricultural Radiology Departtament of Plant Radiobiogy "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 207-213, Article in English THE CREATION AND APPLICATION OF THE LABORATORY COMPLEXES OF
HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS ABSTRACT: In the Institute of Physics, Georgian Academy of Sciences the original instrument of capillary electrophoresis was built. In the work the design modifications of spectrophotometer, providing the effective detection of signals in wide wavelengths are described.The new methods and approaches for preliminary preparation of capillaries for electrophoresis, the techniques of filtration and injection of analyte are proposed. The separation of model protein mixture is carried out. The magnitudes of mobile coefficients are calculated and it is shown that in 8 mM phosphate buffer (V=12 kV) the average number of theoretical plates is changed in interval of 0.95*105- 1.24*105. Georgian Academy of Sciences Institut of Fisics "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 214-223, Article in English ESTIMATIONS OF BACKGROUND RADIATION AND DOSE LOADINGS OF POPULATION IN
GEORGIA ABSTRACT: Radiation background of artificial and natural radionuclides was tuded. Objective geophysical division of Georgian territory into districts was taken into account. The store of cesium-137 in soil is given. The effect of Chernobyl accident is estimated. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 230-242, Article in Russian ESTABLISHMENT OF EXPOSURE DOSES OF GROUND RADIOACTIVITY IN THE
TERRITORY OF GEORGIA ABSTRACT:Ground walking (walkable) gamma-survey has been made all over the territory of Georgia with an observation net 20X20m. A large body of factual material on geological investigations of ore detection of radioactive elements has been summarized. A map was drawn. Exposure doses of background gamma-irradiation were estimated. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 243-259, Article in Georgian TECHNOGENICALLY MODIFIED RADIATION BACKGROUND - A YGIENIC ASPECT ABSTRACT:Technogenic effect as a result of man’s activities (building materials, fertilizers, etc.) is estimated against the radiation background. Material on dose rate estimation both for radioisotopes and artificial radionuclides is provided. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 260-272, Article in Russian THE QESTION OF RADIATION BY RADON AMONG THE POPULATION OF GEORGIA ABSTRACT: In the work is generalized material of radon and its dismiss products contained in the atmosfere in open places, as well as in the buildings built with different buildinga materials on the territory of the Georgia. The avarage concentration of radon is calculated and the doze of radiation among population is established. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 273-276, Article in Russian POPYLLATION OF FOOD STUFF AND RATION WITH RADIONUCLIDE AS A RESULT OF
CHERNOBIL ACCIDENT ABSTRACT: In the work is generalized the data of popyllation of food stuff with radioniclide. stroncium-90 and tsezium-137 and is calculated their mainfenance of daily food ration among the population of Georgia in 1964 -1990. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 277-280, Article in Georgian CREATION OF INFORMATION BASE ON RADIOHYDROGEOECOLOGICAL ANOMALIES OF
GEORGIA ABSTRACT: Massive geological data on the search of radioactive elements were summarized and systematized. On the basis of 100-year material data base on anomalies of natural radiation background was created, a map of radiohydrogeological anomalies is provided. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 281-289, Article in Georgian RADIATION-HYGIENIC ESTIMATION AND EPIDEMIOLOGIC ANALYSIS OF POPULATION
IRRADIATION LEVELS IN THE TRANSCAUCASUS ABSTRACT: Dynamics and structure of dose loading for regions of Georgia are summarized. Epidemiologic information base is created, dynamics of mortality and morbidity for radiation- specific diseases is studied. Tendencies are revealed and prognosis is estimated. Georgian Academy of Sciences Scientific Research Centre of Radiobiology and Radiation Ecology Departtament of Radiation Ecology "Radiation Studies" Vol.8, 1998, Tbilisi, pp. 290-309, Article in Russian |